Enzymes are proteins that put things together or break things apart. So when testing enzymes we are not looking for quantity or weight. Enzymes are best measured by the work they are capable of doing. The potency is usually recorded in the form of an activity unit. Most plant and microbial enzymes are assayed using the Food Chemicals and Codex (FCC) standards. Use of thes recognized assay procedures ensures that the methodology utilized can be reproduced to verify accuracy and legitimacy of the assay.
Performing enzyme assays take years of experience to develop accurate and repeatable results. Our lab has been in business for over 20 years and has been involved in many proficiency testing studies to prove our capabilities and accuracy. We are an ISO 9001:2008 certified and ISO 17025:2005 accredited testing facility. ISO 9001:2008 applies to our quality management system as a whole. ISO 17025:2005 is specific to testing labs, and is very stringent in its requirements. Many of our enzyme assays are included on our ISO 17025:2005 Scope of Accreditation.
Enzyme Assays
|
Unit
Description
|
|
*
|
a-Galactosidase GalU
|
Hydrolysis of p-nitrophenyl-a-D-galactopyranoside
(UV-VIS)
|
*
|
a-Amylase DU
|
Hydrolysis of Lintner-soluble starch (Orbeco Hellige comparator)
|
a-Amylase BAU
|
Hydrolysis of Lintner-soluble starch (Orbeco Hellige comparator)
|
|
b-Glucanase BGU
|
Hydrolysis of Lichenin (UV-VIS spectroscopy)
|
|
Catalase
CatU
|
Hydrolysis of hydrogen peroxide (UV-VIS spectroscopy)
|
|
Cellulase
CU
|
Hydrolysis of CMC (Cannon-Fenske viscometry)
|
|
Diastase
DP
|
Hydrolysis of Lintner-soluble starch (Orbeco Hellige comparator)
|
|
*
|
Glucoamylase
AGU
|
Hydrolysis of p-nitrophenyl-a-D-glucopyranoside
(UV-VIS)
|
Hemicellulase
HCU
|
Hydrolysis of carob gum (Cannon-Fenske viscometry)
|
|
Invertase
SU
|
Hydrolysis of Sucrose (UV-VIS)
|
|
Isolase
IsoU
|
Hydrolysis of p-nitrophenyl-b-D-glucopyranoside
(UV-VIS)
|
|
*
|
Lactase
ALU
|
Hydrolysis of o-nitrophenyl-b-D-galactopyranoside
(UV-VIS)
|
Lipase
FIP
|
Hydrolysis of olive oil (DpH) (Federation
Internationale Pharma)
|
|
Lysozyme
MCG
|
Lysis of Micrococcus lysodeikticus (UV-VIS spectroscopy)
|
|
Nattokinase
FU
|
Hydrolysis of bovine plasma fibrin (UV-VIS spectroscopy)
|
|
Pancreatic
Amylase USP
|
Hydrolysis of soluble starch (TITRN)
|
|
Pancreatic
Lipase USP
|
Hydrolysis of olive oil (DpH)
|
|
Pancreatic
Protease USP
|
Hydrolysis of casein (UV-VIS spectroscopy)
|
|
|
Pepsin
USP
|
Hydrolysis of egg albumin (sediment)
|
*
|
Protease
HUT
|
Hydrolysis of hemoglobin (UV-VIS spectroscopy)
|
Pectinase
PGU
|
Hydrolysis of citrus pectin (Cannon-Fenske viscometry)
|
|
Peptidase
DPPIV
|
Hydrolysis of Gly-Pro p-Nitroanilide (UV-VIS)
|
|
Phytase
FTU
|
Hydrolysis of sodium phytate decahydrate (UV-VIS)
|
|
Protease
AP
|
Hydrolysis of leucine p-nitroanalide (UV-VIS spectroscopy)
|
|
*
|
Protease
FCC PU
|
Hydrolysis of casein @ pH 6.0 (UV-VIS spectroscopy)
|
*
|
Protease
PC
|
Hydrolysis of casein @ pH 7.0 (UV-VIS spectroscopy)
|
*
|
Protease
SAPU
|
Hydrolysis of casein @ pH 3.0 (UV-VIS spectroscopy)
|
Serratiopeptidase
U
|
Hydrolysis of casein @ pH 9.0 (UV-VIS spectroscopy)
|
|
|
Xylanase
XU
|
Hydrolysis of xylose @ pH 5.3 (UV-VIS spectroscopy)
|
*These assays are on out ISO 17025:2005 Scope of Accreditation
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www.soralabs.com
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